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Cedarlane 淋巴細(xì)胞分離柱-相關(guān)技術(shù)文章

更新時間:2024-07-16      瀏覽次數(shù):146

Cedarlane  淋巴細(xì)胞分離柱


Cedarlane公司成立于加拿大,27年間,已經(jīng)逐步發(fā)展成為生命科學(xué)行業(yè)的一家跨國公司,在加拿大和美國擁有超過100名員工。

Cedarlane公司的研究領(lǐng)域。

公司為生命科學(xué)研究人員和臨床醫(yī)生提供無數(shù)的生物和生化產(chǎn)品,提供來自幾乎所有世jie上zui 知 ming 的國際制造商的產(chǎn)品。交貨及時,所有產(chǎn)品都在適當(dāng)?shù)乃铚囟认聝Υ?、接收和運(yùn)輸。員工與客戶和供應(yīng)商密切合作,提供個性化和全面的體驗(yàn),以反映客戶至上的核心價值。通過提供全球超過 600 萬種試劑盒和試劑的門戶,客戶擁有貨運(yùn)整合的優(yōu)勢以及其中固有的便利性和成本節(jié)約;Cedarlane 是一站式商店。

主要分銷產(chǎn)品線包括:抗體、細(xì)胞培養(yǎng)基和過濾、原代細(xì)胞和細(xì)胞系、蛋白質(zhì)和肽、酶和抑制劑、成像和顯微鏡資源、檢測/ELISA試劑盒、精細(xì)化學(xué)品和生化、DNA/RNA分離和純化。

主要制造產(chǎn)品包括:單克隆和多克隆抗體、細(xì)胞分離培養(yǎng)基、組織分型補(bǔ)體和高度表征的神經(jīng)元細(xì)胞系。

Lympholyte®-H是一種密度梯度分離介質(zhì),專門設(shè)計(jì)用于從人外周血和臍帶血中分離淋巴細(xì)胞和單核細(xì)胞。

注意:使用前充分搖晃混勻,靜止2-3min,待氣泡消失后試用。(室溫條件下使用分離液,分離液密度會隨溫度的變化而發(fā)生改變)

樣本制備可使用無血清培養(yǎng)基,減少樣本粘度,更利于分離。

操作步驟:

1.在含有抗凝血劑的試管中收集人血;

2.用等量培養(yǎng)基1:1稀釋血液;

3.根據(jù)不同的方法進(jìn)行分離:

①在離心管中加入3 mL Lympholyte®-H;

②使用移液管,小心地將6 mL稀釋的血液鋪在Lympholyte®-H上,在液面上盡可能少地混合(圖A);

③由于Lympholyte®-H的密度比細(xì)胞懸液更大,因此將形成一個明顯的界面(圖C)。

方法B:

①在離心管中加入6 mL稀釋后的血液。將一個大的(23厘米)移液管放置到試管的底部(圖B);

②慢慢地向移液管中加入Lympholyte®-H,重力使其在稀釋的血液下分層;

③繼續(xù)處理,直到3mL Lympholyte®-H在稀釋的血液下分層。由于Lympholyte®-H比細(xì)胞懸液密度更大,因此會形成明顯的界面(圖C);

4.在室溫下,以800g離心20分鐘;

5.離心后,界面上將有一個明顯的淋巴細(xì)胞層(圖D)。使用移液管,小心地將細(xì)胞吸出,并轉(zhuǎn)移到一個新的離心管中;

6.用培養(yǎng)基稀釋轉(zhuǎn)移的細(xì)胞,以降低溶液的密度。800g離心10分鐘,沉淀淋巴細(xì)胞,然后棄用上清液;

Note:如果需要去除血小板,可在這一步進(jìn)行操作:

方法A

A.加入0.5 mL緩沖液和50 uL凝血酶,然后輕輕重懸細(xì)胞;

B.用緩沖液補(bǔ)足10mL,并充分混合;

C.非常緩慢地(100g)離心細(xì)胞3分鐘;血小板會聚集并沉淀在底部;

D. 取出上清液(包含細(xì)胞),放入另一個試管中,留下聚集的血小板。

方法B

A.800 g離心1分鐘,沉淀細(xì)胞;

B.倒出上清液,用緩沖液重懸細(xì)胞,重復(fù)2次;

C.此時會觀察到,當(dāng)血小板被移除時,產(chǎn)生的上清液會變得更加清晰。

7.在進(jìn)一步處理之前,在培養(yǎng)基中清洗淋巴細(xì)胞2-3次(在這個階段可以使用含有血清的培養(yǎng)基)。

Cedarlane 淋巴細(xì)胞分離柱-相關(guān)技術(shù)文章



以下是Cedarlane公司的熱銷產(chǎn)品

產(chǎn)品名稱

貨號

規(guī)格

Lympholyte®-H Cell Separation Media (Isolation of   lymphocytes from Human peripheral blood), sterile liquid

CL5020

500ml

Baby Rabbit Complement, Tissue Culture Grade -   Frozen

CL3441-S

1ml

Lympholyte®-H Cell Separation Media (Isolation of   lymphocytes from Human peripheral blood), sterile liquid

CL5016

6x100mL

Low-Tox® Guinea Pig Complement, Lyophilized

CL4051

5x1ml

Lympholyte®-M Cell Separation Media (Isolation of   lymphocytes from Mouse lymphoid tissue), sterile liquid

CL5031

100ml

Lympholyte®-Mammal Cell Separation Media (Isolation   of lymphocytes from Mammalian peripheral blood), sterile liquid

CL5115

100ml

Low-Tox®-M Rabbit Complement, Lyophilized

CL3051

5x1ml

Lympholyte®-M Cell Separation Media (Isolation of   lymphocytes from Mouse lymphoid tissue), sterile liquid

CL5030

5x30ml

Lympholyte®-H Cell Separation Media (Isolation of   lymphocytes from Human peripheral blood), sterile liquid

CL5015

100ml

Lympholyte®-H Cell Separation Media (Isolation of   lymphocytes from Human peripheral blood), sterile liquid

CL5026

6x500ml

Anti-Mouse Asialo GM1, Ig Fraction   (Polyclonal)(rabbit Ig)

CL8955

1 mL

Lympholyte®-Mammal Cell Separation Media (Isolation   of lymphocytes from Mammalian peripheral blood), sterile liquid

CL5120

500ml

Human Complement, Pooled, Frozen

CL6600-1

1 ml

Anti-Mouse/Human Mac-2 (Galectin-3), Purified   (Clone M3/38) (rat IgG2a)

CL8942AP

100ug

Anti-Human Tamm Horsfall Purifed (Clone 10.32)   (mouse IgG2b)

CL1032AP

250ug

Anti-Mouse Complement Component C3, FITC (Clone   RmC11H9) (rat IgG2a)

CL7503F

100ug

Lympholyte®-Mammal Cell Separation Media (Isolation   of lymphocytes from Mammalian peripheral blood), sterile liquid

CL5110

5x30ml

Lympholyte®-1.1, sterile liquid

CL6155

500mL

Baby Rabbit Complement, Lyophilized

CL3441

10x1ml

Anti-Human Factor VIII:C HRPO (Polyclonal) (sheep   IgG)

CL20035HP

0.2 mg

Anti-Human Factor VIII:C Aff.Pur.HRPO(Paired Abs)

CL20035K

4 plates

Low-Tox®-H Rabbit Complement, Lyophilized

CL3331

10x1mL

Chicken Gamma Globulin (purified antigen)

CLF802-1

50mg

Lympholyte®-Rat Cell Separation Media (Isolation of   lymphocytes from Rat lymphoid tissue), sterile liquid

CL5041

100mL

Lympholyte®-Rabbit Cell Separation Media (Isolation   of lymphocytes from Rabbit lymphoid tissue), sterile liquid

CL5050

5x30ml

Standard Rabbit Complement, Lyophilized

CL3111

25x1ml

Anti-Rat CD90 (Thy 1.1) Ascites (Clone OX-7) (mouse   IgG1)

CL005A

0.5ml

Rabbit Complement MA, Lyophilized

CL3221

10x1ml

Lympholyte®-poly Cell Separation Media (Isolation of   PMNs from Human peripheral blood), sterile liquid

CL5070

100mL

Anti-Human Fibrinogen Aff.Pur./HRPO (Paired Abs)

CL20067K

5 plates

Lympholyte®-1.1, sterile liquid

CL5095

500mL

O-sialoglycoprotein endopeptidase, Lyophilized

CLE100

1.2mg

Anti-Mouse Thrombocyte (platelet), Antiserum,   (Polyclonal) (rabbit serum)

CLA31440

5mL

Anti-Human Tissue Plasminogen Activator, Affinity   Purified (Polyclonal) (sheep IgG)

CL20115AP

0.5 mg

Anti-Human Factor IX Aff.Pur./HRPO (Paired Abs)

CL20041K

5 plates

Baby Rabbit Complement, Tissue Culture Grade,   Frozen

CL3441-S100

100ml

Standard Guinea Pig Complement, Lyophilized

CL5000

5x5ml

Lympholyte®-H Cell Separation Media (Isolation of   lymphocytes from Human peripheral blood), sterile liquid

CL5010

5x30mL

Human Interleukin-17A

CL101-17-25UG

25ug

Anti-Mouse Collagen Type I, purified IgG

CL50151AP-1

0.1ml

Anti-Mouse CD25 , Purified (Clone PC61.5.3) (rat   IgG1)

CL8925AP

250ug

Anti-Rat IL-10, Purified, (Polyclonal) (rabbit IgG)

CL9583AP

50ug

Lympholyte®-poly Cell Separation Media (Isolation   of PMNs from Human peripheral blood), sterile liquid

CL5071

250 mL

Anti-Mouse PMN, Antiserum, (Polyclonal) (rabbit   serum)

CLA31140

5mL

Anti-Rat Myeloid Lineage, FITC (Clone OX82) (mouse   IgG1)

CL081F

100 ug

Anti-Rat Macrophage, Adsorbed Antiserum,   (Polyclonal) (rabbit serum)

CLAD51240

2mL

Baby Rabbit Complement- Tissue Culture Grade,   Frozen

CL3441-S50

50 ml

Recombinant Human Gastrokine 3

CLPRO2033-2

10ug

Anti-Rat CD45RA/B FITC (Clone OX-33) (mouse IgG1)

CL033F

100ug

Mini Super PAP PEN (tip 1/10" diameter, 3 mL)

MU22

EA

Anti-Rat CD11b/c Purified (Clone OX-42)   (mouse IgG2a)

CL042AP

250ug

Anti-Human Factor H (FITC) (Clone: OX-24)   (mouse IgG1)

CL7672F

100 ug

Anti-Mouse I-Ad, Ascites (Clone 34-5-3S)   (mouse IgG2a)

CL8713A

0.5 mL

Anti-Human Antithrombin III Affinity   Purified (Polyclonal) (sheep IgG)

CL20015AP

0.5mg

Cedarlane/Anti-Rat CD45, 10 nm Gold   Conjugate,OD3 (Clone OX-1) (mouse lgG1)

CL009G

0.5 mL

Cedarlane/Rat IL-2ELISA kit(precoated)

CL00113K-96

96T




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